Staphylococcus microbes in the bovine skin microbiome attract blood-feeding stable flies

The human skin microbiome reportedly contributes to the attraction of mosquitoes hosts. We tested hypothesis that bovine microbes affect blood-feeding stable flies, Stomoxys calcitrans , their Microbes were collected from a calf and adult cow, subsequently isolated identified by mass spectrometry genetic sequencing. Separate groups ( i ) four Staphylococcus congeners S. chromogenes sciuri simulans, succinus ii three bacterial heterogeners Glutamicibacter protophormiae Corynebacterium stationis Wautersiella sp.) grown on agar, each attracted flies in still-air olfactometers, as did congener singly. also room bioassays. In greenhouse bioassays with paired black barrels visual (surrogate host) stimuli, treatment barrel baited agar induced significantly more fly alighting responses than control sterile agar. This effect could not be demonstrated cattle farm, possibly because chemically visually complex surroundings. Ammonia emitted synthetic blend microbe odorants enhanced attractiveness ammonia. Optimal likely requires integration multimodal host cues.